In this proposal, we plan to define new methods dedicated to the analysis of nD microscopy data and to the modeling of molecular and macromolecular mechanisms at the cell level. In combination with the amount of information provided by high-throughput nD microscopy and automated image analysis methods (object segmentation, tracking, ...), we aim at designing computational and statistical models to understand membrane trafficking and, more precisely to better elucidate the role of Rab family proteins inside their multiprotein complexes. Our main objective is then to provide computational methods and mathematical models to automatically extract, organize and model dynamic information observed in temporal series of images in multi-dimensional (nD) microscopy. This represents a real scientific challenge in applied mathematics, computer science and biology.

Nevertheless and beyond, we believe that our studies will help to describe and better define the dynamic architecture of multi-protein complexes involved in the time and space regulation of many essential molecular mechanisms of the living, such as the dynamic organization of the cytoskeleton, cytokinesis, nuclear envelop biogenesis, cell adhesion,...

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